You will typically require the following sample quantities for acceptable results:
- 200 MHz - ca. 5 mg
- 400 MHz - ca. 2 mg for 1H, or 20 mg for 13C
When handling oils, the quantity drawn up into a pipette by capillary action should be sufficient for a 1H spectrum.
Service samples are most often run on the NEO600, which is equipped with a cryogenic probe and is thus substantially more sensitive than the open-access machines. This allows us to handle samples which are very dilute, going down to sub-milligram quantities.
Nevertheless, it is still in your best interests to submit as much sample as can be spared — this means that you are more likely to get your data back faster, and will also yield spectra of higher quality which are easier to interpret.